Journal: Immunity, Inflammation and Disease

Article Title: Paeoniflorin Ameliorates Spinal Cord Injury by Controlling Apoptosis and Ferroptosis in H 2 O 2 ‐Damaged PC12 Cells

doi: 10.1002/iid3.70324

Figure Lengend Snippet: Paeoniflorin promotes SIRT3 expression in H₂O₂‐damaged PC12 cells. (A) RT‐qPCR analysis of SIRT3 mRNA expression; (B and C) western blotting analysis of SIRT3 protein expression. Data are presented as mean ± SD ( n = 3). *** p < 0.001, **** p < 0.0001 versus control group; ## p < 0.01, ### p < 0.001 versus H₂O₂ group.

Article Snippet: To establish an oxidative stress injury model, PC12 cells were pretreated with 300 μM hydrogen peroxide (H2O2; Sigma‐Aldrich, USA) for 24 h. After H2O2 exposure, cells were treated with different concentrations of paeoniflorin (25, 50, and 100 μM; MedChemExpress, USA) for an additional 24 h. In specific experiments, cells were co‐treated with paeoniflorin (100 μM) and the sirtuin 3 (SIRT3) inhibitor 3‐TYP (30 μM; Selleck Chemicals, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Immunity, Inflammation and Disease

Article Title: Paeoniflorin Ameliorates Spinal Cord Injury by Controlling Apoptosis and Ferroptosis in H 2 O 2 ‐Damaged PC12 Cells

doi: 10.1002/iid3.70324

Figure Lengend Snippet: SIRT3 inhibitor 3‐TYP suppresses paeoniflorin‐induced upregulation of SIRT3 expression. (A) RT‐qPCR analysis of SIRT3 mRNA expression; (B and C) western blotting analysis of SIRT3 protein expression. Data are presented as mean ± SD ( n = 3). * p < 0.05, **** p < 0.0001 versus H₂O₂ group; ## p < 0.01, #### p < 0.0001 versus H₂O₂ + paeoniflorin group.

Article Snippet: To establish an oxidative stress injury model, PC12 cells were pretreated with 300 μM hydrogen peroxide (H2O2; Sigma‐Aldrich, USA) for 24 h. After H2O2 exposure, cells were treated with different concentrations of paeoniflorin (25, 50, and 100 μM; MedChemExpress, USA) for an additional 24 h. In specific experiments, cells were co‐treated with paeoniflorin (100 μM) and the sirtuin 3 (SIRT3) inhibitor 3‐TYP (30 μM; Selleck Chemicals, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Immunity, Inflammation and Disease

Article Title: Paeoniflorin Ameliorates Spinal Cord Injury by Controlling Apoptosis and Ferroptosis in H 2 O 2 ‐Damaged PC12 Cells

doi: 10.1002/iid3.70324

Figure Lengend Snippet: Paeoniflorin regulates apoptosis in H₂O₂‐damaged PC12 cells via SIRT3. (A) Cell viability assessed by MTT assay; (B and C) apoptosis rate analyzed by flow cytometry; (D–F) western blot analysis of Bax and Bcl‐2 protein expression. Data are presented as mean ± SD ( n = 3). *** p < 0.001, **** p < 0.0001 versus H₂O₂ group; ## p < 0.01, #### p < 0.0001 versus H₂O₂ + paeoniflorin group.

Article Snippet: To establish an oxidative stress injury model, PC12 cells were pretreated with 300 μM hydrogen peroxide (H2O2; Sigma‐Aldrich, USA) for 24 h. After H2O2 exposure, cells were treated with different concentrations of paeoniflorin (25, 50, and 100 μM; MedChemExpress, USA) for an additional 24 h. In specific experiments, cells were co‐treated with paeoniflorin (100 μM) and the sirtuin 3 (SIRT3) inhibitor 3‐TYP (30 μM; Selleck Chemicals, USA).

Techniques: MTT Assay, Flow Cytometry, Western Blot, Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Paeoniflorin Ameliorates Spinal Cord Injury by Controlling Apoptosis and Ferroptosis in H 2 O 2 ‐Damaged PC12 Cells

doi: 10.1002/iid3.70324

Figure Lengend Snippet: Paeoniflorin regulates ferroptosis in H₂O₂‐damaged PC12 cells via SIRT3. (A) Immunofluorescence detection of intracellular ROS accumulation (Magnification: 200×, bar = 100 μm); (B–D) ELISA assays for Cys, GSH, and GPX4 levels. Data are presented as mean ± SD ( n = 3). ** p < 0.01, *** p < 0.001 versus H₂O₂ group; ## p < 0.01, ### p < 0.001 versus H₂O₂ + paeoniflorin group.

Article Snippet: To establish an oxidative stress injury model, PC12 cells were pretreated with 300 μM hydrogen peroxide (H2O2; Sigma‐Aldrich, USA) for 24 h. After H2O2 exposure, cells were treated with different concentrations of paeoniflorin (25, 50, and 100 μM; MedChemExpress, USA) for an additional 24 h. In specific experiments, cells were co‐treated with paeoniflorin (100 μM) and the sirtuin 3 (SIRT3) inhibitor 3‐TYP (30 μM; Selleck Chemicals, USA).

Techniques: Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Pharmacology

Article Title: Improved myocardial mitochondrial energy metabolism in rats with chronic heart failure by modifying fatty acid oxidation using an extract of sand-fired aconite (Jianchang gang processing)

doi: 10.3389/fphar.2025.1600410

Figure Lengend Snippet: Effects of captopril and different processed aconite products on the AMPK, PGC-1α, and SIRT3 pathway in the myocardial tissue of CHF rats. (A) AMPK Western blot images. (B) Relative AMPK protein expression in the myocardial tissue of rats in each group ( n = 3). (C) p-AMPK Western blot images. (D) Relative p-AMPK protein expression in the myocardial tissue of rats in each group ( n = 3). (E) PGC-1α Western blot images. (F) Relative PGC-1α protein expression in the myocardial tissue of rats in each group ( n = 3). (G) SIRT3 Western blot images. (H) Relative SIRT3 protein expression in the myocardial tissue of rats in each group ( n = 3). # P < 0.05 and ## P < 0.01 in comparisons with the control group; * P < 0.05, ** P < 0.01, and *** P < 0.001 in comparisons with the CHF model group.

Article Snippet: Sirtuin 3 (SIRT3) antibodies (Batch No. 10099-1-AP), peroxisome proliferator-activated receptor coactivator-1α (PGC-1α) antibodies (Batch No. 66369-1-Ig), and carnitine palmitoyl transferase 1 (CPT1) antibodies (Batch No. 15184-1-AP) were purchased from Wuhan Sanying Biotechnology Co., Ltd. Atrial natriuretic peptide (ANP) Kits (Batch No. MK6949A), brain natriuretic peptide (BNP; Batch No. MK1608A), norepinephrine (NE; Batch No. MK2097A), malondialdehyde (MDA; Batch No. MK6877A), superoxide dismutase (SOD; Batch No. MK1927A), sodium-potassium-ATPase (Na + -k + -ATPase; Batch No. MK6748A), calcium-magnesium-ATPase (Ca 2+ -Mg 2+ -ATPase; Batch No. MK0007RA), adenosine triphosphate (ATP; Batch No. MK6913A), and free fatty acid (FFA; Batch No. MK1865A) kits were purchased from Jiangsu Sumeike Biotechnology Co., Ltd. We used a Fresco17 refrigerated centrifuge (Thermo Fisher company), Vevo2100 ultra-high resolution small animal color doppler ultrasound real-time imaging system (Visual Sonics company), Paraffin embedding machine, EG1150 (Leica company), Paraffin slicer RM2016 (Leica company), MF43 Microscope (Mingmei photoelectric technology Co., Ltd.), MC50 digital imaging measurement and analysis system (Mingmei photoelectric technology Co., Ltd.), Infinite F50 automatic enzyme label analyzer (Tecan company), Ultra-low temperature refrigerator (Haier company), and JEM-1400 transmission electron microscope (JEOL company) for the study.

Techniques: Western Blot, Expressing, Control

Journal: Frontiers in Pharmacology

Article Title: Improved myocardial mitochondrial energy metabolism in rats with chronic heart failure by modifying fatty acid oxidation using an extract of sand-fired aconite (Jianchang gang processing)

doi: 10.3389/fphar.2025.1600410

Figure Lengend Snippet: SFAS improves DOX-induced CHF via the AMPK-PGC-1α-SIRT3 pathway.

Article Snippet: Sirtuin 3 (SIRT3) antibodies (Batch No. 10099-1-AP), peroxisome proliferator-activated receptor coactivator-1α (PGC-1α) antibodies (Batch No. 66369-1-Ig), and carnitine palmitoyl transferase 1 (CPT1) antibodies (Batch No. 15184-1-AP) were purchased from Wuhan Sanying Biotechnology Co., Ltd. Atrial natriuretic peptide (ANP) Kits (Batch No. MK6949A), brain natriuretic peptide (BNP; Batch No. MK1608A), norepinephrine (NE; Batch No. MK2097A), malondialdehyde (MDA; Batch No. MK6877A), superoxide dismutase (SOD; Batch No. MK1927A), sodium-potassium-ATPase (Na + -k + -ATPase; Batch No. MK6748A), calcium-magnesium-ATPase (Ca 2+ -Mg 2+ -ATPase; Batch No. MK0007RA), adenosine triphosphate (ATP; Batch No. MK6913A), and free fatty acid (FFA; Batch No. MK1865A) kits were purchased from Jiangsu Sumeike Biotechnology Co., Ltd. We used a Fresco17 refrigerated centrifuge (Thermo Fisher company), Vevo2100 ultra-high resolution small animal color doppler ultrasound real-time imaging system (Visual Sonics company), Paraffin embedding machine, EG1150 (Leica company), Paraffin slicer RM2016 (Leica company), MF43 Microscope (Mingmei photoelectric technology Co., Ltd.), MC50 digital imaging measurement and analysis system (Mingmei photoelectric technology Co., Ltd.), Infinite F50 automatic enzyme label analyzer (Tecan company), Ultra-low temperature refrigerator (Haier company), and JEM-1400 transmission electron microscope (JEOL company) for the study.

Techniques: